Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity.
نویسندگان
چکیده
Applying directed evolution to the phloroglucinol synthase PhlD from Pseudomonas fluorescens Pf-5 has provided the first example of engineering enhanced productivity in a type III polyketide synthase, and a rare instance of improving the activity of a biosynthetic enzyme from secondary metabolism.
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ورودعنوان ژورنال:
- Molecular bioSystems
دوره 4 3 شماره
صفحات -
تاریخ انتشار 2008